Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Vila, R.; Benito, A.; Ribo, M.; Vilanova, M.
    Mapping the stability clusters in bovine pancreatic ribonuclease A (2009), Biopolymers, 91, 1038-1047.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression in Escherichia coli strain BL21 Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 D53A slight stabilization, increases the helix propensity of alpha-helix 3 Bos taurus
4.6.1.18 F120A destabilization Bos taurus
4.6.1.18 F46A equivalent destabilization for F46A and F46L variants Bos taurus
4.6.1.18 F46L equivalent destabilization for F46A and F46L variants Bos taurus
4.6.1.18 F8A substitution of Phe8 results in a recombinant variant significantly destabilized Bos taurus
4.6.1.18 F8L substitution of Phe8 results in a recombinant variant significantly destabilized Bos taurus
4.6.1.18 K7A slight stabilization, increases the helix propensity of alpha-helix 1 Bos taurus
4.6.1.18 L35A less destabilizing than M30A substitution Bos taurus
4.6.1.18 L51A shortening the side chain of the hydrophobic solvent-exposed residue Leu51 to Ala has almost no effect in the stability of ribonuclease A Bos taurus
4.6.1.18 M13A critical position for the ribonuclease A stability Bos taurus
4.6.1.18 M30A critical position for the ribonuclease A stability Bos taurus
4.6.1.18 M79A a minor role in the stabilization of the protein Bos taurus
4.6.1.18 Q28A promotes an increase in the helix propensity, from 0.99 in the wild-type to 1.5 Bos taurus
4.6.1.18 T87A no effect in the protein stability Bos taurus
4.6.1.18 V43A substitution of the hydrophobic residue valin 43 by alanin results in an increase in the global stability of the ribonuclease A structure of 4.02 kJ/mol in free energy Bos taurus
4.6.1.18 Y25A critical position for the ribonuclease A stability Bos taurus
4.6.1.18 Y25F decreases the stability of the enzyme by 7.41 kJ/mol Bos taurus
4.6.1.18 Y73A a minor role in the stabilization of the protein Bos taurus
4.6.1.18 Y97A substitution of Tyr97 results in a variant significantly destabilized Bos taurus
4.6.1.18 Y97L substitution of Tyr97 results in a variant significantly destabilized Bos taurus

General Stability

EC Number General Stability Organism
4.6.1.18 calculation of thermodynamic parameters from thermally induced unfolding curves Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.18 13590
-
 mutant enzyme Y73A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13590
-
 mutant enzyme Y97A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13600
-
 mutant enzyme Y25A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13610
-
 mutant enzyme F120A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13610
-
 mutant enzyme F46A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13610
-
 mutant enzyme F8A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13620
-
 mutant enzyme K7A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13620
-
 mutant enzyme M13A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13620
-
 mutant enzyme M30A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13620
-
 mutant enzyme Q28A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13620
-
 mutant enzyme T87A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13630
-
 mutant enzyme Y97L, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13640
-
 mutant enzyme D53A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13640
-
 mutant enzyme L35A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13640
-
 mutant enzyme L51A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13650
-
 mutant enzyme F46L, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13650
-
 mutant enzyme F8L, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13650
-
 mutant enzyme M79A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13660
-
 mutant enzyme V43A, MALDI-TOF/TOF mass spectrometry Bos taurus
4.6.1.18 13670
-
 mutant enzyme Y25F, MALDI-TOF/TOF mass spectrometry Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus P61823
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 ribonuclease A
-
 Bos taurus
4.6.1.18 RNase A
-
 Bos taurus